FastQCFastQC Report
Tue 7 May 2024
EGAF00007822388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9797
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTA6897.0327651321833216No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2052.09247728896601No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1671.7046034500357254No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1051.0717566602021027No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACA720.7349188527100133No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.6940900275594569No Hit
CTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTCGG550.561396345820149No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTG380.38787383893028476No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC380.38787383893028476No Hit
CTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTC370.3776666326426457No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAA370.3776666326426457No Hit
CCTTCAACCTGCTCATGGGTAGGTCACATGGTTTCGGGTCTACGTCATGA280.28580177605389406No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA280.28580177605389406No Hit
GCTTGAGACACATCAGATTCCTCCATTTGATTATTTTCCTCAGAAGTCTC270.27559456976625496No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG260.2653873634786159No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC250.25518015719097686No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.25518015719097686No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC250.25518015719097686No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC230.23476574461569868No Hit
ATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTCGGTGG220.2245585383280596No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACTGTGTA210.21435133204042053No Hit
GTGTGACTGCACCAGCAGTGGTCAGGTCCCTGTACTCCCGATACATGTTG200.20414412575278149No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.19393691946514238No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.19393691946514238No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAA180.18372971317750333No Hit
CTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGTTCTCGG180.18372971317750333No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGA170.17352250688986423No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCAC160.16331530060222518No Hit
GGTCACATGGTTTCGGGTCTACGTCATGATACTAATTCGCCCTGTTCAGA160.16331530060222518No Hit
CGGTAGCACTGTGTGACTGCACCAGCAGTGGTCAGGTCCCTGTACTCCCG160.16331530060222518No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC150.15310809431458608No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG150.15310809431458608No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC150.15310809431458608No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGGGTA140.14290088802694703No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGGA140.14290088802694703No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGA140.14290088802694703No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG140.14290088802694703No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA140.14290088802694703No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA140.14290088802694703No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT130.13269368173930796No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAG130.13269368173930796No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA130.13269368173930796No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC130.13269368173930796No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG120.12248647545166887No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG120.12248647545166887No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC110.1122792691640298No Hit
CAATTTCCCCGGATGACTTCTTCATCTTCTTCAGCTGTGACACAAAGTAC110.1122792691640298No Hit
CATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTCGGTGGTCGCC110.1122792691640298Illumina Single End PCR Primer 1 (100% over 22bp)
GCTAAGGATCGTCGCCTTGGTAGGCTTTTACCCCACCAACTAGCTAATCC100.10207206287639074No Hit
GTCATAGCGCAGCCAGATGCCAAAGTTCTTCACACGCAGGGGTGACTTCT100.10207206287639074No Hit
GAACTACTACTTACAGGGCCTTCGTTTCCACTACCTTCAGAATGCAGCAA100.10207206287639074No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA100.10207206287639074No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT100.10207206287639074No Hit
CTGTGTGACTGCACCAGCAGTGGTCAGGTCCCTGTACTCCCGATACATGT100.10207206287639074No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)