FastQCFastQC Report
Tue 7 May 2024
EGAF00007822391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8828
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCG4114.655641141821477No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCT2843.2170367014046213No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1231.393294064340734No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1211.3706388763026733No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC800.9062075215224286No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC550.6230176710466697No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG500.5663797009515179No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTAC470.5323969188944269No Hit
ATTGCGCAATGTGATTATAGGGGAGAATAAACAACGAAGACTCTTGACCA460.5210693248753965No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGAT450.5097417308563661No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT430.4870865428183053No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAA380.43044857272315357No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT330.3738106026280018No Hit
ATTGCGCAATGGGATGTGAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTAT320.3624830086089715No Hit
ATTGCGCAATGGCTCCCGAGGGATGAACTCTCTTGAATTTATTGCAACGC300.33982782057091077No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCG260.29451744449478934No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC240.2718622564567286No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC240.2718622564567286No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT230.26053466243769824No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG220.24920706841866785No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC220.24920706841866785No Hit
ATTGCGCAATGCCAAGAAGGGGGGGTGAAAGGCTAAACAAACCTGGAAAT210.2378794743996375No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA190.21522428636157678No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG190.21522428636157678No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT190.21522428636157678No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG180.20389669234254643No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC170.1925690983235161No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC170.1925690983235161No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT160.18124150430448574No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCGCG150.16991391028545538No Hit
CGTCTGTTGTGTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAA130.14725872224739467No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCT130.14725872224739467No Hit
ATTGCGCAATGAGATGGCCGGAAAGGTCTCCTGAGCAAGGCTCGTCCCCT130.14725872224739467No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA120.1359311282283643No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT120.1359311282283643No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGAGCG120.1359311282283643No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC120.1359311282283643No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC120.1359311282283643No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT110.12460353420933393No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTA110.12460353420933393No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG110.12460353420933393No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTCCGATCG110.12460353420933393No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT100.11327594019030357No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAA100.11327594019030357No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC100.11327594019030357No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT100.11327594019030357No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA100.11327594019030357No Hit
ATTGCGCAATGCGCGCACGGGGGTTGTCAATAAAGCGTTGATCCGAGGAT90.10194834617127321No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGAGCT90.10194834617127321No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG90.10194834617127321No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTCCGATCT90.10194834617127321No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT90.10194834617127321No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)