FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8828
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTA7698.710919800634345No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1641.8577254191209787No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1011.1440869959220663No Hit
CTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGG891.0081558676937017No Hit
CTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTC680.7702763932940644No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.724966017217943No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCT550.6230176710466697No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTG470.5323969188944269No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.5210693248753965No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAAT370.41912097870412324No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGTCGCC370.41912097870412324Illumina Single End PCR Primer 1 (95% over 22bp)
ATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGG350.39646579066606247No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC280.31717263253285005No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG270.30584503851381967No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG240.2718622564567286No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG230.26053466243769824No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.24920706841866785No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGA210.2378794743996375No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT200.22655188038060714No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG190.21522428636157678No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAA190.21522428636157678No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA180.20389669234254643No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC170.1925690983235161No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC160.18124150430448574No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGGCGCC150.16991391028545538Illumina RNA PCR Primer (95% over 21bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA140.15858631626642503No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC140.15858631626642503No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.15858631626642503No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATC130.14725872224739467No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGTGGCC120.1359311282283643Illumina RNA PCR Primer (95% over 21bp)
CTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGTTCTCGG120.1359311282283643No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGC120.1359311282283643No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1359311282283643No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAAT110.12460353420933393No Hit
GTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.12460353420933393No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA100.11327594019030357No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA100.11327594019030357No Hit
ATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGGGG90.10194834617127321No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA90.10194834617127321No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA90.10194834617127321No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA90.10194834617127321No Hit
TTTTTGTGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAAT90.10194834617127321No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)