FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8073
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCG3594.446921838226186No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCT2082.576489533011272No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1692.0933977455716586No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1632.0190759321194105No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1111.3749535488665925No Hit
ATTGCGCAATGTCAAGAAGGGGGCTTTCCGAGCTGCTAGCGCTCCTGCGA720.8918617614269788No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC430.5326396630744457No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG390.4830917874396135No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCAT370.45831784962219746No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT330.4087699739873653No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTG320.39638300507865726No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC310.3839960361699492No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGC280.3468351294438251No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA270.33444816053511706No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT260.322061191626409No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT240.29728725380899296No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCG230.2849002849002849No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC190.23535240926545273No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG190.23535240926545273No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA170.21057847144803665No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC140.17341756472191255No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCAGCG140.17341756472191255No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT130.1610305958132045No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCT130.1610305958132045No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGC120.14864362690449648No Hit
ATTGCGCAATGATTCCTCTGGGAGTAAGAAAGAGCACACGGGGGATGCCT120.14864362690449648No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC120.14864362690449648No Hit
ATTGCGCAATGATATTATGGGAAGCACCGGGCGCACCATGCGTTGGATGA120.14864362690449648No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCA120.14864362690449648No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTG120.14864362690449648No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG110.13625665799578843No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA110.13625665799578843No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCGCG110.13625665799578843No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC110.13625665799578843No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT100.12386968908708039No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT100.12386968908708039No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG100.12386968908708039No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT90.11148272017837235No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCCTCG90.11148272017837235No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.11148272017837235No Hit
CTCCTTGGGGTGGGGGTGGAAAAACAGACCGGGGTTCCCGTGTGCCTGAG90.11148272017837235No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT90.11148272017837235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)