FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8073
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTA6427.952434039390561No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2072.564102564102564No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1441.7837235228539576No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.9909575126966431No Hit
CTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGG650.8051529790660225No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.7927660101573145No Hit
CTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTC630.7803790412486065No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGTCGCC520.644122383252818Illumina Single End PCR Primer 1 (95% over 22bp)
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT420.5202526941657376No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG420.5202526941657376No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCT400.49547875634832156No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.4211569428960733No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAAT330.4087699739873653No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTG330.4087699739873653No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.3468351294438251No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG260.322061191626409No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC250.309674222717701No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG240.29728725380899296No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC230.2849002849002849No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA230.2849002849002849No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG210.2601263470828688No Hit
ATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGG200.24773937817416078No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC180.2229654403567447No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.2229654403567447No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC170.21057847144803665No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA170.21057847144803665No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC170.21057847144803665No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGTCGGC160.19819150253932863Illumina RNA PCR Primer (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGA160.19819150253932863No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC150.18580453363062058No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGGCGCC140.17341756472191255Illumina RNA PCR Primer (95% over 21bp)
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAAT130.1610305958132045No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGGCGGC120.14864362690449648No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT120.14864362690449648No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA120.14864362690449648No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGGGGTCGCC120.14864362690449648Illumina RNA PCR Primer (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA120.14864362690449648No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA110.13625665799578843No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC110.13625665799578843No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA100.12386968908708039No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAA100.12386968908708039No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATC90.11148272017837235No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG90.11148272017837235No Hit
TTTTTTTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG90.11148272017837235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)