FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6762
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCG2283.3717834960070983No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCT2153.1795326826382726No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1281.892931085477669No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1271.878142561372375No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC921.3605442176870748No Hit
ATTGCGCAATGATATGTTGGGGGGATCAAGCGACTAAGTGCATATGGTGG741.0943507837917774No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGC470.6950606329488317No Hit
ATTGCGCAATGCACACCGCGGGGAAATAGCCCCTCCATTATAGACCGTAC460.6802721088435374No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG410.6063294883170659No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAAT310.45844424726412303No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG300.44365572315882873No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC290.4288671990535345No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC260.3845016267376516No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT220.3253475303164744No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT210.3105590062111801No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC190.28098195800059156No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC190.28098195800059156No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT180.26619343389529726No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCG150.22182786157941436No Hit
ATTGCGCAATGCGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAG140.2070393374741201No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA140.2070393374741201No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCACTCG130.1922508133688258No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA130.1922508133688258No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC120.1774622892635315No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT110.1626737651582372No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG110.1626737651582372No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA110.1626737651582372No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC110.1626737651582372No Hit
ATTGCGCAATGATTACACGGGGGGGTGGAAAGGAAAAAAAAAGGTAATTG100.14788524105294293No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG90.13309671694764863No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT90.13309671694764863No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT90.13309671694764863No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCGGT90.13309671694764863No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG90.13309671694764863No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.13309671694764863No Hit
ATTGCGCAATGGCGAGGACGGGACAGGAAGAAAAAGGATACCAAGAAAGA90.13309671694764863No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG90.13309671694764863No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC90.13309671694764863No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT90.13309671694764863No Hit
ATTGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.11830819284235432No Hit
ATTGCGCAATGCGCGCGGGGGTGATGAAGGACGGGACTAACACCGATATG80.11830819284235432No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCGGG80.11830819284235432No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG80.11830819284235432No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCT80.11830819284235432No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC80.11830819284235432No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG80.11830819284235432No Hit
ATTGCGCAATGGGGCCGAGGGGTGCGCCGACGGCAGACGGGCGGAAAGGC70.10351966873706005No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCACTCT70.10351966873706005No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC70.10351966873706005No Hit
ATTGCGCAATGCGAGCAGGGGGTAGCCTCGGAAGTAAGAATGATGGAGGT70.10351966873706005No Hit
ATTGCGCAATGGGCCGCCGGGGTGGTTCTCTCCGAAATAGCTTTAGGGCT70.10351966873706005No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGG70.10351966873706005No Hit
ATTGCGCAATGATAGCAGGGGGTAGCCTCGGAAGTAAGAATGATGGAGGT70.10351966873706005No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG70.10351966873706005No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)