FastQCFastQC Report
Tue 7 May 2024
EGAF00007822400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6762
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTA4787.0689145223306715No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1852.735876959479444No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1161.715468796214138No Hit
CTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGG670.9908311150547175No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.9168884945282461No Hit
CTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTC570.8429458740017746No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCT510.7542147293700089No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.6654835847382431No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGTCGCC430.6359065365276546Illumina Single End PCR Primer 1 (95% over 22bp)
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTG310.45844424726412303No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC300.44365572315882873No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT300.44365572315882873No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAAT290.4288671990535345No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG260.3845016267376516No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG240.354924578527063No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG190.28098195800059156No Hit
ATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGG190.28098195800059156No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT180.26619343389529726No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC170.25140490979000296No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGA170.25140490979000296No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.23661638568470864No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC140.2070393374741201No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.1922508133688258No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG120.1774622892635315No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA120.1774622892635315No Hit
GCATACGCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG120.1774622892635315No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1774622892635315No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGTGGCC110.1626737651582372Illumina RNA PCR Primer (95% over 21bp)
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA110.1626737651582372No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA110.1626737651582372No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC100.14788524105294293No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAAT90.13309671694764863No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC90.13309671694764863No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA90.13309671694764863No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGGCGCC80.11830819284235432Illumina RNA PCR Primer (95% over 21bp)
ATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGGGG80.11830819284235432No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA80.11830819284235432No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA80.11830819284235432No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAAT80.11830819284235432No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAA80.11830819284235432No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGTGGGC70.10351966873706005No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA70.10351966873706005No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA70.10351966873706005No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGGGTA70.10351966873706005No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC70.10351966873706005No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)