FastQCFastQC Report
Tue 7 May 2024
EGAF00007822401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7920
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2062.601010101010101No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCG1942.44949494949495No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1551.9570707070707072No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1521.9191919191919191No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCT1351.7045454545454544No Hit
ATTGCGCAATGTAAACTGTGGGAAGATTGAAAAACAAGCTATTCTTGAAG740.9343434343434343No Hit
ATTGCGCAATGAGTGGTCGGGGACGTCATCTACATCGAACGAGTCCAGCG700.8838383838383838No Hit
ATTGCGCAATGGGACCCAAGGGGCTTCTCTTTCTTCCTGTCTGGCTGGAA660.8333333333333334No Hit
ATTGCGCAATGACGTGTACGGGGTTGTGGGTGAGTGAGTGATCCTGTACT580.7323232323232324No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG410.5176767676767677No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTG370.4671717171717171No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTA370.4671717171717171No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC310.39141414141414144No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCG290.3661616161616162No Hit
ATTGCGCAATGAGAGGGTAGGGAAGCTAGAAGCACACTCCTGGAGAGTGA290.3661616161616162No Hit
ATTGCGCAATGCACTGCCTGGGAAGGTTGGGTCAGGAAATAAGACAGAAA280.35353535353535354No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAAT270.3409090909090909No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG210.2651515151515152No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT190.2398989898989899No Hit
ATTGCGCAATGGAGAATTGGGGAGGTTAATATTCCTGTACTTCTGGTTAT190.2398989898989899No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA180.22727272727272727No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCT170.21464646464646464No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC170.21464646464646464No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC140.17676767676767677No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA120.15151515151515152No Hit
ATTGCGCAATGAGTCAGATGGGGCGTGGCGTCAGTCCAGGCAGACTTGGG120.15151515151515152No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG110.1388888888888889No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT110.1388888888888889No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTT110.1388888888888889No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA110.1388888888888889No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT110.1388888888888889No Hit
ATTGCGCAATGTGGGGATTGGGGACAGAGAGCGAAAACAAAAAAAAAAAC110.1388888888888889No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG100.12626262626262627No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG100.12626262626262627No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC100.12626262626262627No Hit
ATTGCGCAATGTGGGGATTGGGGACAGAGAGCGAAAACAAAAAAAAAAAA100.12626262626262627No Hit
ATTGCGCAATGTGCAGTGGGGGTGGATACGAGCAGTTGTCAAAAACAACC90.11363636363636363No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTG90.11363636363636363No Hit
ATTGCGCAAGCAGAAGACGGCATACGAGATTCTGACTGTGACTGGAGTTC90.11363636363636363RNA PCR Primer, Index 40 (96% over 29bp)
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGGTAAATCG90.11363636363636363No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT90.11363636363636363No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC80.10101010101010101No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT80.10101010101010101No Hit
ATTGCGCAATGTGGCAAGAGGGGGGGCGCCAATATGAAAAAAGAATCTAA80.10101010101010101No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC80.10101010101010101No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGGTAAATCT80.10101010101010101No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATGG80.10101010101010101No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)