FastQCFastQC Report
Tue 7 May 2024
EGAF00007822407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9320
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCT3523.776824034334764No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCG2642.832618025751073No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1421.5236051502145922No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1191.2768240343347639No Hit
ATTGCGCAATGCGACTAACGGGAAGCCCCATCTCATCCTGGCACGCCCTA770.8261802575107295No Hit
ATTGCGCAATGAGATCCGAGGGGATGACACGGTACAAAATGGTAGGAGCG770.8261802575107295No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC650.6974248927038627No Hit
ATTGCGCAATGTGTATAAAGGGCAAATGTTCCCTCAGTGATCCTTTTACT580.6223175965665236No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG540.5793991416309012No Hit
ATTGCGCAATGAAGGAGGTGGGGGCCATCGCCGCAGATCCAGCGCCCAGA530.5686695278969957No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTAC510.5472103004291845No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC460.4935622317596567No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAA390.4184549356223176No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT340.3648068669527897No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGAT330.3540772532188841No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC290.3111587982832618No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT270.2896995708154506No Hit
ATTGCGCAATGGAGAACACGGGGGGTGAGTGAGTGATCCTGTACTGTCGA270.2896995708154506No Hit
ATTGCGCAATGCATTCGCGGGGAGTTGGGTTTAATACGACGTGAGTCAGT270.2896995708154506No Hit
ATTGCGCAATGCAGGTTGGGGGAGAGCAGGAGGGGCCGGAGTATTGGGAC260.27896995708154504No Hit
ATTGCGCAATGTCGAGCGCGGGGAGTTGGGTTTAATACGACGTGAGTCAG240.2575107296137339No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA230.24678111587982834No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT220.23605150214592274No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGAGCT210.22532188841201717No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC210.22532188841201717No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG200.2145922746781116No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC190.20386266094420602No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT190.20386266094420602No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGAGCG180.19313304721030042No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCT170.18240343347639484No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC170.18240343347639484No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCGCG170.18240343347639484No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA160.17167381974248927No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTCCGATCG160.17167381974248927No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG160.17167381974248927No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTA150.1609442060085837No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAA150.1609442060085837No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCG150.1609442060085837No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA150.1609442060085837No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG150.1609442060085837No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.15021459227467812No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC140.15021459227467812No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCTCG140.15021459227467812No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT140.15021459227467812No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT130.13948497854077252No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA120.12875536480686695No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC120.12875536480686695No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGGTGCCT110.11802575107296137No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT110.11802575107296137No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT110.11802575107296137No Hit
ATTGCGCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAG100.1072961373390558No Hit
ATTGCGCAATGATATTATGGGGAGATCAAAACCAACCCGGTCAGCCCCTC100.1072961373390558No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)