FastQCFastQC Report
Tue 7 May 2024
EGAF00007822408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9320
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1611.7274678111587984No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC810.8690987124463521No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTA660.7081545064377682No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.27896995708154504No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC160.17167381974248927No Hit
CATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGTGGTCGCC150.1609442060085837Illumina Single End PCR Primer 1 (95% over 23bp)
ATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGTGG130.13948497854077252No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.13948497854077252No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130.13948497854077252No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG130.13948497854077252No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGG100.1072961373390558No Hit
GTCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTA100.1072961373390558No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)