FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7624
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTA5957.804302203567682No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1892.4790136411332635No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1001.3116474291710387No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT821.0755508919202519No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGG610.8001049317943336No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.7607555089192025No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGG540.7082896117523609No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGGGTA370.48530954879328436No Hit
CATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGTGGTCGCC370.48530954879328436Illumina Single End PCR Primer 1 (95% over 23bp)
CTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTC370.48530954879328436No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGT350.4590766002098636No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTG340.4459601259181532No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC280.36726128016789084No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG250.3279118572927597No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT250.3279118572927597No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.3147953830010493No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA200.26232948583420773No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG190.24921301154249736No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC180.236096537250787No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA170.2229800629590766No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.2229800629590766No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG160.2098635886673662No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA150.1967471143756558No Hit
ATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGTGG140.18363064008394542No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCGTGGGTGTA140.18363064008394542No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.18363064008394542No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG130.17051416579223505No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC130.17051416579223505No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC130.17051416579223505No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC120.15739769150052466No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC120.15739769150052466No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC110.14428121720881426No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA110.14428121720881426No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGA100.13116474291710387No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGTTCTCGG100.13116474291710387No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGT100.13116474291710387No Hit
CATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGTGGTGGGC100.13116474291710387No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA100.13116474291710387No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT90.1180482686253935No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA90.1180482686253935No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG90.1180482686253935No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC90.1180482686253935No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATC90.1180482686253935No Hit
CATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGTGGGCGGC80.1049317943336831No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT80.1049317943336831No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGGCTTGGGTGTA80.1049317943336831No Hit
CATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGTGGGGGCC80.1049317943336831No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCG80.1049317943336831No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA80.1049317943336831No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA80.1049317943336831No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)