FastQCFastQC Report
Tue 7 May 2024
EGAF00007822413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13007
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGACGCATTATCT3632.790804951180134No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGACGCATTATCG3522.706235104174675No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2972.283385869147382No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2381.8297839624817407No Hit
ATTGCGCAATGATGATACCGGGGAAGGTATACTACATCGCAAGATGAGCA2151.6529561005612363No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1971.5145690781886676No Hit
ATTGCGCAATGCGATGCACGGGAAGAGAATGTTACCACCTGAACAGTCCT1461.1224725147997232No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGACGCAT960.738064119320366No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC680.522795417851926No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG610.46897824248481584No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGA550.422849235027293No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGACGCATTAT550.422849235027293No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGACGCAGTATCG520.39978473129853154No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC520.39978473129853154No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT510.3920965633889444No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT480.369032059660183No Hit
ATTGCGCAATGATGGCCCCGGGGGCCAATCAAACCGGGAAATAGCTGGTT480.369032059660183No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCGACGCATTATCG450.34596755593142153No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT400.3075267163834858No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC350.2690858768355501No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG310.2383332051972015No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG260.19989236564926577No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCGACGCATTATCT250.19220419773967864No Hit
ATTGCGCAATGGAGGAAAGGGGGAAAAATATAAAACCAACATTACAACAT250.19220419773967864No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC240.1845160298300915No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGAC240.1845160298300915No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA240.1845160298300915No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGACGCA230.17682786192050434No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA230.17682786192050434No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT220.1691396940109172No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC220.1691396940109172No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGACGCAGTATCT210.16145152610133007No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG210.16145152610133007No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA200.1537633581917429No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG190.14607519028215576No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGA190.14607519028215576No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGACGCATTAGCT180.1383870223725686No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT180.1383870223725686No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC180.1383870223725686No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT180.1383870223725686No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT170.1306988544629815No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGACGCATTAGCG170.1306988544629815No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT170.1306988544629815No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT170.1306988544629815No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT160.12301068655339432No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT150.11532251864380717No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGACGCATGATCT140.10763435073422004No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGACGCATTATCTCG140.10763435073422004No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC140.10763435073422004No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG140.10763435073422004No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)