FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13007
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTA8906.84246943953256No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3913.0060736526485736No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2912.2372568616898594No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.9072038133312832No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGG1040.7995694625970631No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGG1000.7688167909587146No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.6765587760436688No Hit
CTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTC690.530483585761513No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT640.4920427462135773No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGGGTA610.46897824248481584No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTG600.4612900745752287No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGT540.41516106711770584No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG420.32290305220266013No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG390.2998385484738987No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG380.29215038056431153No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.261397708925963No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC330.25370954101637583No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC330.25370954101637583No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA320.24602137310678865No Hit
CATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGTGGTCGCC320.24602137310678865Illumina Single End PCR Primer 1 (95% over 23bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA310.2383332051972015No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.2383332051972015No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.2383332051972015No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA270.20758053355885292No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC250.19220419773967864No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTG240.1845160298300915No Hit
ATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGTGG230.17682786192050434No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG230.17682786192050434No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT220.1691396940109172No Hit
ACTCAGGATACTGCTATTACTTATTTATCTTACCTATACGGGACTATCAC220.1691396940109172No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGA200.1537633581917429No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTC200.1537633581917429No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC200.1537633581917429No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA190.14607519028215576No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGT190.14607519028215576No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA190.14607519028215576No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA180.1383870223725686No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC180.1383870223725686No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA160.12301068655339432No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGTTCTCGG150.11532251864380717No Hit
ATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGGGG150.11532251864380717No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTT150.11532251864380717No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC140.10763435073422004No Hit
CATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGTGGTGGCC140.10763435073422004No Hit
CTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGTTCTC140.10763435073422004No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG140.10763435073422004No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)