FastQCFastQC Report
Tue 7 May 2024
EGAF00007822417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8764
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCT3954.507074395253309No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCG2422.761296211775445No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1822.07667731629393No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1681.9169329073482428No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1241.4148790506617983No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAAT570.6503879507074395No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG500.5705157462345961No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTA450.5134641716111364No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC410.4678229119123688No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT320.3651300775901415No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT270.3080785029666819No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTG270.3080785029666819No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC260.29666818804198997No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT230.2624372432679142No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC220.25102692834322227No Hit
ATTGCGCAATGCAAAGAGGGGGCTGGCTACGCGCGGCGCCTGCGCTCCCA210.23961661341853036No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA200.22820629849383844No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCG200.22820629849383844No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC200.22820629849383844No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG180.2053856686444546No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTG160.18256503879507074No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG150.1711547238703788No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG150.1711547238703788No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA150.1711547238703788No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCT140.1597444089456869No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA140.1597444089456869No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGC130.14833409402099498No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC130.14833409402099498No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGGTAAATCT130.14833409402099498No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC120.13692377909630307No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.13692377909630307No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT120.13692377909630307No Hit
ATTGCGCAATGGAAGGGGAGGGCATAAAAAGCAAACAAAAAACGGACACA120.13692377909630307No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT110.12551346417161113No Hit
ATTGCGCAATGGGCATGACGGGGACCACCGCCTTCCTGCATCCTCCTCAG110.12551346417161113No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGGTAAATCG110.12551346417161113No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG110.12551346417161113No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCTCG110.12551346417161113No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT110.12551346417161113No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC100.11410314924691922No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTT100.11410314924691922No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG100.11410314924691922No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCT100.11410314924691922No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAAGCG90.1026928343222273No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG90.1026928343222273No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT90.1026928343222273No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGCTGTCTCTTA90.1026928343222273No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG90.1026928343222273No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT90.1026928343222273No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGG90.1026928343222273No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)