FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1203
Sequences flagged as poor quality0
Sequence length151
%GC63

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT17814.796342477140481No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC786.483790523690773No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC221.828761429758936No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151.2468827930174564No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTTGTGGGGGGGGGGG70.5818786367414797No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.3325020781379884No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGGTGGGGGGGGGGGGG40.3325020781379884No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC40.3325020781379884No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGTGTAGATCTCGGTGGTCGCC30.24937655860349126Illumina RNA PCR Primer (95% over 22bp)
ATGTTTGTAACAGTACAGTGAAACTAAAGGTCAGAGACAATGGCTGTCAG30.24937655860349126No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.24937655860349126No Hit
ACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.24937655860349126No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.24937655860349126No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGTTGTTGTGGGGGGGGGGG30.24937655860349126No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG30.24937655860349126No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGTAGTTGGAGGGGGGGGGG20.1662510390689942No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGTGGTGATGTGGG20.1662510390689942No Hit
ATACACATCTGACGCTGCCGACGAGAAGACCGCTGGGTTGTTGTGGGGGG20.1662510390689942No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTTGGGGGGGGGGGGG20.1662510390689942No Hit
GTGTATAAGAGACAGATTGCGCACTCTGTCTCTTATACACATCTGACGCT20.1662510390689942No Hit
GTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1662510390689942No Hit
GTTTGTAACAGTACAGTGAAACTAAAGGTCAGAGACAATGGCTGTCAGTT20.1662510390689942No Hit
GTGTATAAGAGACCGATTGCGCAATCTGTCTCTTATACACATCTGACGCT20.1662510390689942No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC20.1662510390689942No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT20.1662510390689942No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTTTTGTGGGGGGGGGGG20.1662510390689942No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGTGTAGATCACGGTGGTCGCC20.1662510390689942Illumina Single End PCR Primer 1 (95% over 21bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATGT20.1662510390689942No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA20.1662510390689942No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTTGTCGGGGGGGGGG20.1662510390689942No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCAGACGCT20.1662510390689942No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGTTGATGTGGGGGGGGGGG20.1662510390689942No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1662510390689942No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGGTGTGGGGGGGGGGC20.1662510390689942No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGAAAA20.1662510390689942No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGTGTATATCTCGGTGGTCGCC20.1662510390689942Illumina Single End PCR Primer 1 (95% over 21bp)
ATACACATCTGACGCTGCCGACGAGAAGACCGCTGTGTAGATATCGGTGG20.1662510390689942No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATG20.1662510390689942No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACAAGAAGACCGCGGGGTT20.1662510390689942No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC20.1662510390689942No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA20.1662510390689942No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG20.1662510390689942No Hit
CTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGTGGTGTTGTG20.1662510390689942No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)