FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734
Sequences flagged as poor quality0
Sequence length151
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1729.91926182237601No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC925.3056516724336795No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.922722029988466No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC100.5767012687427913No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC100.5767012687427913No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.5190311418685121No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG90.5190311418685121No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGTTGTTGTGGGGGGGGGGG60.34602076124567477No Hit
ATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTTGTGGGGGG60.34602076124567477No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTTGTGGG50.28835063437139563No Hit
ATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG50.28835063437139563No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTTGTGGGGGGGGGGG50.28835063437139563No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGA50.28835063437139563No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCT40.2306805074971165No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA40.2306805074971165No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATCTGTC40.2306805074971165No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA40.2306805074971165No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2306805074971165No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2306805074971165No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG40.2306805074971165No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG40.2306805074971165No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGTGTT40.2306805074971165No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCG30.17301038062283738No Hit
TGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGC30.17301038062283738No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTTGGGGG30.17301038062283738No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC30.17301038062283738No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGGGGTGTTGTGGGGGGGGGGG30.17301038062283738No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT30.17301038062283738No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGTGTAGATCTCGGTGGTCGCC30.17301038062283738Illumina RNA PCR Primer (95% over 22bp)
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT30.17301038062283738No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA30.17301038062283738No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGG30.17301038062283738No Hit
GGTGCACGACAGCTCAGGTCCCCCTTATCCCAAAGTTACGGGGGCATTTT30.17301038062283738No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA30.17301038062283738No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGGTGTGGGGGGGGGGG30.17301038062283738No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA30.17301038062283738No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGGGTTGTTGTGGG30.17301038062283738No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGTT30.17301038062283738No Hit
CTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGGGTTGGTGTG30.17301038062283738No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGA30.17301038062283738No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA30.17301038062283738No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGGGGTGTTGTCGGGGGGGGGG20.11534025374855825No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGGGGTGTTATCGGGGGGGGGG20.11534025374855825No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGTA20.11534025374855825No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAATGT20.11534025374855825No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATATGACGCT20.11534025374855825No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTTGGGGGGGGGGGGG20.11534025374855825No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGGGTT20.11534025374855825No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGTCT20.11534025374855825No Hit
GTGTATAAGAGACCGATTGCGCAATCTGTCTCTTATACACATCTGACGCT20.11534025374855825No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAATG20.11534025374855825No Hit
GCCTACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11534025374855825No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA20.11534025374855825No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20.11534025374855825No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGTTTTTGTGGG20.11534025374855825No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGTAT20.11534025374855825No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGTGTAGATATCGG20.11534025374855825No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGTTTTTGTGGGGGGGGGGG20.11534025374855825No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGGG20.11534025374855825No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGGGGTGGTGTGGGGGGGGGGG20.11534025374855825No Hit
GTATAAGAGACAGATTGCGCAATGGGTAACCCGGGAGCGACATTGCGCAA20.11534025374855825No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTTGTCGG20.11534025374855825No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATCT20.11534025374855825No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA20.11534025374855825No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT20.11534025374855825No Hit
GTCGTTTCCCCCTGGACTCTGCGCCCCTCACCGCCACCACACCGCACGGG20.11534025374855825No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA20.11534025374855825No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGT20.11534025374855825No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGGGGTGTGGTGGG20.11534025374855825No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGAA20.11534025374855825No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGG20.11534025374855825No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA20.11534025374855825No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAAA20.11534025374855825No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA20.11534025374855825No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTTGTGTGGGGGGGGGGG20.11534025374855825No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11534025374855825No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTTTTGTGGGTGGGGGGG20.11534025374855825No Hit
CTCGTATGCCGTCTTCTGCTTGCGCAATCTGTCTCTTATACACATCTGAC20.11534025374855825Illumina Single End Adapter 1 (100% over 22bp)
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAACTGTAT20.11534025374855825No Hit
ATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGTTGTTGTGGGGGG20.11534025374855825No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT20.11534025374855825No Hit
CTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTTGGG20.11534025374855825No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC20.11534025374855825No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGTA20.11534025374855825No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGGGGTGTTGGCGGGGGGGGGG20.11534025374855825No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC20.11534025374855825No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC20.11534025374855825No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGGGTTGTTGTAGG20.11534025374855825No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC20.11534025374855825No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA20.11534025374855825No Hit
CTACAGCCTCGACCTGCTGGGTTCAAGTATTCTTCCCACCTCAGCTTACC20.11534025374855825No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG20.11534025374855825No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC20.11534025374855825No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG20.11534025374855825No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGGGGTGTTGGGGGGGGGGGGG20.11534025374855825No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCAAAAATTT20.11534025374855825No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGA20.11534025374855825No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC20.11534025374855825No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)