FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250
Sequences flagged as poor quality0
Sequence length151
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1139.04No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC403.2No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC161.28No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.8No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.4No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGGGGT50.4No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA50.4No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.4No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT40.32No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAATGT30.24No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30.24No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT30.24No Hit
CTATTTACGTCCTCTTTAACTATTGTTTTTTTTTCTACTTTTTTTCCCTT30.24No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTTGTGGGGGGGGGGG30.24No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA30.24No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATTT30.24No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGTGTAGATCTCGG30.24No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC30.24No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC30.24No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA30.24No Hit
TCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA20.16No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGGGTTTTTGTCGGGGGGGGGG20.16No Hit
GTTTTCATTAGTGTGTTCTCGCGATCATTGTTTTGTGCATTTCTCTCTAT20.16No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGGTGTCGG20.16No Hit
ATACACATCTGACGCTGCCGACGAGAAGACCGCGGTGTTGTTGTGGGGGG20.16No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGGGGTGTGGGGGGGGGGG20.16No Hit
CATCTGACGCTGCCGACGAGAATACCGCTGTGTATATATCGGTGGTCGCC20.16No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGTAT20.16No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGGGTT20.16No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT20.16No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCA20.16No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA20.16No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATTTAT20.16No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA20.16No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTTTTGGGGGGGGGGGGG20.16No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA20.16No Hit
CTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGTGGTTGTG20.16No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTTTTGTGGGGGGGGGGG20.16No Hit
TCACAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA20.16No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGTGTAGATCTCGGTGGTCGCC20.16Illumina RNA PCR Primer (95% over 22bp)
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAAAATTG20.16No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGGGGTGGGGG20.16No Hit
GCATACGCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG20.16No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGGTGGGGGGGGGGGGG20.16No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGTTGTTGTCGGGGGGGGGG20.16No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGA20.16No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAACTGT20.16No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.16No Hit
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.16No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA20.16No Hit
GTATAAGAGACAGATTGCGCCCATTGCGCAATCTGTCTCTTATACACATC20.16No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA20.16No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGGTGTGGGGGGGGGGG20.16No Hit
CTATAGGGTCTTCTCGTCTTTTATTAAAATTTTAGCTTTCTAACTAAAAA20.16No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGGGGTGTGGG20.16No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAATA20.16No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATG20.16No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGTT20.16No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCA20.16No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG20.16No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG20.16No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG20.16No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAA20.16No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGTGTT20.16No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)