FastQCFastQC Report
Tue 7 May 2024
EGAF00007822443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10275
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATCG3833.727493917274939No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATCT2342.2773722627737225No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2172.111922141119221No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2082.0243309002433088No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1391.3527980535279804No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC620.6034063260340633No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG530.5158150851581509No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACAT460.44768856447688565No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC440.4282238442822385No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT430.41849148418491483No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT370.36009732360097324No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCAT350.34063260340632606No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAG320.31143552311435524No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT300.291970802919708No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATCG290.2822384428223844No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG230.22384428223844283No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC230.22384428223844283No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAG210.20437956204379565No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA200.19464720194647203No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA180.1751824817518248No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT180.1751824817518248No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG180.1751824817518248No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG170.16545012165450124No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCAGCG170.16545012165450124No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGG160.15571776155717762No Hit
ATTGCGCAATGGGCAGGGGGGGAGGGAGTAAGATCCTCATCAATAAATGG160.15571776155717762No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC140.1362530413625304No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA140.1362530413625304No Hit
ATTGCGCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGG130.1265206812652068No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC130.1265206812652068No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATCGCG130.1265206812652068No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.11678832116788322No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATGG120.11678832116788322No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAA120.11678832116788322No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT110.10705596107055962No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT110.10705596107055962No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT110.10705596107055962No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG110.10705596107055962No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)