FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10275
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGTA2972.8905109489051095No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2622.5498783454987834No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1741.6934306569343065No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGTT1461.4209245742092458No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1451.4111922141119222No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGGA1391.3527980535279804No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGGT1211.1776155717761556No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.6131386861313868No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA600.583941605839416No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTC490.4768856447688564No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.4282238442822385No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTG390.3795620437956204No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.30170316301703165No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTA290.2822384428223844No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC290.2822384428223844No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG280.2725060827250608No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC280.2725060827250608No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG250.24330900243309003No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG250.24330900243309003No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTA210.20437956204379565No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA210.20437956204379565No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG200.19464720194647203No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC200.19464720194647203No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC180.1751824817518248No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA170.16545012165450124No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA170.16545012165450124No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC170.16545012165450124No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC150.145985401459854No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACT140.1362530413625304No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA130.1265206812652068No Hit
TCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTA130.1265206812652068No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT130.1265206812652068No Hit
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.1265206812652068No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.1265206812652068No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA120.11678832116788322No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAA120.11678832116788322No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG120.11678832116788322No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGT120.11678832116788322No Hit
CATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTAT110.10705596107055962No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTAT110.10705596107055962No Hit
CAGCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG110.10705596107055962No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)