FastQCFastQC Report
Tue 7 May 2024
EGAF00007822445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13722
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGACGCATTATCG5514.015449642909196No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGACGCATTATCT3372.455910217169509No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2932.135257251129573No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2201.6032648301996792No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1871.3627751056697275No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGACGCAT1010.7360443084098528No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGA920.6704562017198659No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG770.5611426905698877No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGACGCATTAT760.5538551231598892No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC710.5174172861098965No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT570.4153913423699169No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCGACGCATTATCG550.4008162075499198No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT530.38624107272992275No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGACGCAGTATCG480.34980323567993005No Hit
ATTGCGCAATGAGCGACCGGGGCGCGATAAGGGCGTTTGGTGGATGCCTT450.3279405334499344No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT410.2987902638099402No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC370.26963999416994605No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT370.26963999416994605No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGA330.2404897245299519No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC330.2404897245299519No Hit
ATTGCGCAATGCTAGGGGCGGGGGGGCGAGGAGAAAAGGCGCAGGAATAT310.22591458970995482No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC310.22591458970995482No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC310.22591458970995482No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG300.21862702229995626No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC280.2040518874799592No Hit
ATTGCGCAATGCACCCCGTGGGGGTAGGGGAGGCGCAGGGAAACCGAGTC270.19676432006996064No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG270.19676432006996064No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG260.1894767526599621No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG250.18218918524996355No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG240.17490161783996502No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGAC230.16761405042996647No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG230.16761405042996647No Hit
ATTGCGCAATGGGGAATGGGGGGGATAATATTTCAGGAGTTCGAGACCAG230.16761405042996647No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGACGCA220.16032648301996794No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA220.16032648301996794No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCGACGCATTATCT220.16032648301996794No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGACGCATTAGCG210.1530389156099694No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA210.1530389156099694No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT210.1530389156099694No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC200.14575134819997085No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT200.14575134819997085No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG190.1384637807899723No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGACGCATTATCGCG190.1384637807899723No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT180.13117621337997376No Hit
ATTGCGCAATGCATCGCCGGGGGGGCGAGGAGAAAAGGCGCAGGAATATG170.12388864596997523No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCG160.11660107855997667No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT150.10931351114997813No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT150.10931351114997813No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT150.10931351114997813No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT150.10931351114997813No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.1020259437399796No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT140.1020259437399796No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT140.1020259437399796No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)