FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7701
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCG3043.947539280612907No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCT2333.025581093364498No Hit
ATTGCGCAATGGTGGGCAGGGGGGCATCCGTGCCCGGCTCAGGCCCATCG1572.0386962732112712No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1421.8439163744968186No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC991.2855473315153876No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC720.9349435138293729No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG530.6882223087910662No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAAT410.5323983898195039No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGC400.5194130632385404No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT350.4544864303337229No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG280.3635891442669783No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC280.3635891442669783No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC240.3116478379431243No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT230.29866251136216077No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCACTCG220.28567718478119725No Hit
ATTGCGCAATGGTGAACGCGGAACAGAAACATTCGAAGATCCGCACCGAC180.23373587845734323No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT170.22075055187637968No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCG170.22075055187637968No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC160.20776522529541616No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA160.20776522529541616No Hit
ATTGCGCAATCACTTATGGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAA140.18179457213348915No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA140.18179457213348915No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG130.16880924555252563No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCGCG130.16880924555252563No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG120.15582391897156214No Hit
ATTGCGCAATGTGCTGTTCGGGGGTTAAGTCCCGCAACGAGCGCAACCCT120.15582391897156214No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC110.14283859239059862No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG100.1298532658096351No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCT100.1298532658096351No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC100.1298532658096351No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGG90.11686793922867161No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT90.11686793922867161No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT90.11686793922867161No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGATG90.11686793922867161No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC90.11686793922867161No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT90.11686793922867161No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT80.10388261264770808No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT80.10388261264770808No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT80.10388261264770808No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCGGT80.10388261264770808No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)