FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7701
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGTA2753.5709648097649658No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGTT1221.5842098428775482No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1221.5842098428775482No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGGA1171.5192832099727307No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC941.22062069861057No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGGT861.116738085962862No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT811.0518114530580445No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.7921049214387741No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTC460.5973250227243215No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTG450.584339696143358No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG370.4804570834956499No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC320.4155304505908323No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.40254512400986886No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTA290.3765744708479418No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG280.3635891442669783No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG250.3246331645240878No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA240.3116478379431243No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA240.3116478379431243No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.2467212050383067No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG180.23373587845734323No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT170.22075055187637968No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC170.22075055187637968No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA160.20776522529541616No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA150.19477989871445267No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG150.19477989871445267No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC150.19477989871445267No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC140.18179457213348915No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA130.16880924555252563No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.16880924555252563No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC130.16880924555252563No Hit
GGGGAAGGCAGCCCGAGGGCTCTTTCTCCCAGGAAGTTTCTGGGCAGCTG120.15582391897156214No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC120.15582391897156214No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTA120.15582391897156214No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAATGT100.1298532658096351No Hit
GGTCAGAGCTCAGGGAGGCCGAGGCTTCCTCTTGTTTTCCGTGGCCGACT100.1298532658096351No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA100.1298532658096351No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA100.1298532658096351No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC100.1298532658096351No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC100.1298532658096351No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA90.11686793922867161No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGA90.11686793922867161No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA90.11686793922867161No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA90.11686793922867161No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGT90.11686793922867161No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAA80.10388261264770808No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA80.10388261264770808No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT80.10388261264770808No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA80.10388261264770808No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT80.10388261264770808No Hit
GCTCAGGGAGGCCGAGGCTTCCTCTTGTTTTCCGTGGCCGACTGTCATCC80.10388261264770808No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)