FastQCFastQC Report
Tue 7 May 2024
EGAF00007822451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11927
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCG3803.186048461473966No Hit
ATTGCGCAATGGGCAACCGGGGGCCTTTCCGATCCGCCATCGTGGGTGGA3192.6746038400268297No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2952.4733797266705793No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCT2402.012241133562505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1891.5846398926804728No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1871.567871216567452No Hit
ATTGCGCAATGGAGCGGGTGGGAGTAGATGGTATTAGAGTGAGAATTTAA1541.2911880607026076No Hit
ATTGCGCAATGACGTACCAGGGGGACCACCATGCTGGCTCTTCGTACTTC1351.1318856376289093No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGAT730.612056678125262No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGAT610.5114446214471368No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC610.5114446214471368No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG600.5030602833906263No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA590.4946759453341159No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC430.36052653642994886No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT350.29345183197786534No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC340.2850674939213549No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA320.26829881780833403No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT320.26829881780833403No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT300.2515301416953131No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT290.24314580363880273No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCG260.2179927894692714No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC260.2179927894692714No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA250.20960845141276097No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAG240.20122411335625054No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAGAGATGATCG240.20122411335625054No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG230.19283977529974008No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA220.18445543724322966No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC210.1760710991867192No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGA210.1760710991867192No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG210.1760710991867192No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA190.15930242307369832No Hit
ATTGCGCAATGGGGAAAAGGGGGAAGTATATTGTAATTGTTTAATCTTAG180.1509180850171879No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG180.1509180850171879No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG170.14253374696067744No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAGAGATGATCT170.14253374696067744No Hit
ATTGCGCAATGCCGGGGGGGGGGATAAAAAGAAATGGAAAGTGAAAAGTC160.13414940890416702No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC160.13414940890416702No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT150.12576507084765656No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT150.12576507084765656No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT150.12576507084765656No Hit
ATTGCGCAATGGGCGAGGTGGGAGTAGATGGTATTAGAGTGAGAATTTAA140.11738073279114615No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT140.11738073279114615No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG140.11738073279114615No Hit
ATTGCGCAATGATCCGTGGGGGGGGATAAAAAGAAATGGAAAGTGAAAAG130.1089963947346357No Hit
ATTGCGCAATCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA120.10061205667812527No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCTCG120.10061205667812527No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)