FastQCFastQC Report
Tue 7 May 2024
EGAF00007822452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11927
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3853.2279701517565185No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGTA3723.1189737570218834No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2402.012241133562505No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGTT1881.5762555546239625No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGGA1441.2073446801375032No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGGT1291.0815796092898466No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.9977362287247422No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.7797434392554708No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTC800.6707470445208351No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTG680.5701349878427098No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTA580.48629160727760545No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG460.38567955059948017No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA440.3689108744864593No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG380.31860484614739665No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.31860484614739665No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG360.3018361700343758No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC350.29345183197786534No Hit
GTGTAAGACTTCTTCTTCCTTTTCTTAGCACCACCCCGAAGTCTCAACAC300.2515301416953131No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC300.2515301416953131No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.24314580363880273No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC280.2347614655822923No Hit
CATTTTCCCCCATCCATCTCTCCTACTTGTCTTCTTTGTATTTGTTCTAA280.2347614655822923No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACT280.2347614655822923No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC280.2347614655822923No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC270.22637712752578182No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA250.20960845141276097No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTAT240.20122411335625054No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA230.19283977529974008No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC230.19283977529974008No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG220.18445543724322966No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTA210.1760710991867192No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA190.15930242307369832No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT190.15930242307369832No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC190.15930242307369832No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGT190.15930242307369832No Hit
ATGTTGTAGTCAGACAAAGTCCGGCCATCTTCCAGCTGCTTACCAGCAAA170.14253374696067744No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA160.13414940890416702No Hit
GATGAAGGGTGGACTCCTTTTGAATGTTGTAGTCAGACAAAGTCCGGCCA150.12576507084765656No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA150.12576507084765656No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA140.11738073279114615No Hit
GCCTCTGCTGATCAGGAGGAATTCCTTCCTTATCCTGGATCTTGGCCTTT140.11738073279114615No Hit
GGGTGGACTCCTTTTGAATGTTGTAGTCAGACAAAGTCCGGCCATCTTCC140.11738073279114615No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA140.11738073279114615No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA130.1089963947346357No Hit
CTTCAGTACACAACTTCTCCAGTGTTATTATACCCGCCCAACACATTTTC130.1089963947346357No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.1089963947346357No Hit
TCTTAGCACCACCCCGAAGTCTCAACACCAGATGAAGGGTGGACTCCTTT120.10061205667812527No Hit
CTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGTAGATCTCGG120.10061205667812527No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT120.10061205667812527No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAATGT120.10061205667812527No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)