FastQCFastQC Report
Tue 7 May 2024
EGAF00007822453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3074
Total Bases464.1 kbp
Sequences flagged as poor quality0
Sequence length151
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC31310.18217306441119No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1063.4482758620689653No Hit
ATTGCGCAATGTTGCTTTCGGGGGGTGAAATAAGTGGAGGTCCGAACCGA491.594014313597918No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG331.073519843851659No Hit
ATTGCGCAATGATGTACATGGGGGGGGTTGCAGTGACCAGGCCCGGGCGA110.357839947950553No Hit
ATTGCGCAATGAGACAGTGGGGATACACACACTTATGCTGAGAACTGCAG100.32530904359141183No Hit
GGGGGGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80.2602472348731295No Hit
ATTGCGCAATGACGGGCCCGGGGAAGGTGCTGTGTTTGGCCGTCGCTGTT70.2277163305139883No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.16265452179570591No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.16265452179570591No Hit
ATTGCGCAATGAGCCATAAGGGGGGGGTCGCAGTGACCAGGCCCGGGCGA40.13012361743656475No Hit
ATTGCGCAAACTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC40.13012361743656475No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC40.13012361743656475No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)