FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3074
Sequences flagged as poor quality0
Sequence length151
%GC63

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT41813.597918022121014No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1755.692908262849707No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC481.5614834092387768No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGT240.7807417046193884No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTT140.4554326610279766No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTA140.4554326610279766No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.3903708523096942No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG100.32530904359141183No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG90.29277813923227064No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAG80.2602472348731295No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA70.2277163305139883No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGG70.2277163305139883No Hit
CTATTATTTTGAGGTCATATCTAGTATTCAGAGTTTGCCTCGATTTGGTA70.2277163305139883No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGGTGGTCGCC70.2277163305139883Illumina RNA PCR Primer (95% over 23bp)
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTCGG70.2277163305139883No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC60.1951854261548471No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGGGGGGGGGG60.1951854261548471No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA60.1951854261548471No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGG60.1951854261548471No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTGGCGGGGGGGGGG50.16265452179570591No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.16265452179570591No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGGGGGGGGGC50.16265452179570591No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGGG50.16265452179570591No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTG50.16265452179570591No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTCACCGGGGCTTCGGTCGCC50.16265452179570591No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGA50.16265452179570591No Hit
CCTTTATCACTGAGCGGTCATTTAGGGGCCTTAGCTGGTGATCCGGGCTG40.13012361743656475No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTGGGGGGGGGGG40.13012361743656475No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT40.13012361743656475No Hit
CTCCGAGACAGTGCCCAGATCGTTACGCCTTTCGTGCGGGTCGGAACTTA40.13012361743656475No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGGGGGGGGGGGGG40.13012361743656475No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTTGTTGTC40.13012361743656475No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG40.13012361743656475No Hit
CTTTCAAGGATGGCTGCTTCTAGGCAAACCTCCTGGCTGTCTCTGCACCC40.13012361743656475No Hit
CTTCTAGGCAAACCTCCTGGCTGTCTCTGCACCCCTACCTCCTTTATCAC40.13012361743656475No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG40.13012361743656475No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTG40.13012361743656475No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA40.13012361743656475No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.13012361743656475No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTCGG40.13012361743656475No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTGTGGGGGGGGGGG40.13012361743656475No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTGGG40.13012361743656475No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA40.13012361743656475No Hit
CATATCTAGTATTCAGAGTTTGCCTCGATTTGGTACCGCTCTCGCGGCCC40.13012361743656475No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC40.13012361743656475No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)