FastQCFastQC Report
Tue 7 May 2024
EGAF00007822455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11516
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCG5154.472038902396665No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCT3072.6658562000694683No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3012.6137547759638764No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1651.432789162903786No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1171.0159777700590484No Hit
ATTGCGCAATGTTCGAGCCGGGAGGGGAGCGTCGTGTCAGTGGTGAAGCT990.8596734977422715No Hit
ATTGCGCAATGTGAGTCTTGGGGTGCTGACTGAGTACCGTGGCCTGACCG730.633900659951372No Hit
ATTGCGCAATGGGGTTTACGGGGCGCGAGTGGCGGAATGGAAAAGAAAAA640.5557485237929837No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTC590.5123306703716568No Hit
ATTGCGCAATGGAGTCGGGGGGGGTACAGTGAAACTGCGAATGGCTCATT580.5036470996873914No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA560.48627995831886067No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAAT550.47759638763459533No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG530.4602292462660646No Hit
ATTGCGCAATGAGTCGGGGGGGGTACAGTGAAACTGCGAATGGCTCATTA490.4254949635290032No Hit
ATTGCGCAATGCAGAATACGGGATAGCGACGTTGCTTTTTGATCCTTCGA490.4254949635290032No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT420.3647099687391455No Hit
ATTGCGCAATGTCATGAACGGGGGGGAGCCCACGGTTGTGGGTGAGTGAG420.3647099687391455No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC410.3560263980548802No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC390.33865925668634944No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT380.32997568600208405No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT360.3126085446335533No Hit
ATTGCGCAATGAGAACGTGGGGGAGCCCACGGTTGTGGGTGAGTGAGTGA350.30392497394928797No Hit
ATTGCGCAATGCGGGCTAGGGGGACTCGTCGATGGACGCCAAGCCAAGCT320.27787426189649184No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCG310.2691906912122265No Hit
ATTGCGCAATGCACGCGTCGGGGAGCGAGGTGGCGGGTCCGCCCGTACCC290.2518235498436957No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC250.21708926710663426No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG230.1997221257381035No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA230.1997221257381035No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA210.18235498436957276No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC210.18235498436957276No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG210.18235498436957276No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA200.1736714136853074No Hit
ATTGCGCAATGTGATAGACGGGCTGAAAATTATCAAGGAAAAAACAATTA190.16498784300104202No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA190.16498784300104202No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT180.15630427231677665No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGT180.15630427231677665No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC160.13893713094824592No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT160.13893713094824592No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG160.13893713094824592No Hit
ATTGCGCAATGTTGTGGGCGGGAGAACAAGAGCACGGAGCGTGCCAAGAT150.13025356026398055No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCGCG150.13025356026398055No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAAGCG140.12156998957971517No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC140.12156998957971517No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG140.12156998957971517No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCCATCG140.12156998957971517No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAG130.11288641889544981No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG130.11288641889544981No Hit
ATTGCGCAATGTGTAGGGGGGGGGAGTGAAGCTGCTCATCGAGATGGAGC130.11288641889544981No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGG120.10420284821118443No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG120.10420284821118443No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCT120.10420284821118443No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATGG120.10420284821118443No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)