FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10185
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCG3953.8782523318605793No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCT3052.9945999018163967No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2262.218949435444281No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1701.6691212567501228No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1021.0014727540500736No Hit
ATTGCGCAATGTGCACCAAGGGGGAGGCGAGTCATGGACTGTGTATTTTG880.8640157093765342No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTG630.6185567010309279No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC500.49091801669121254No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC460.4516445753559155No Hit
ATTGCGCAATGCGTCCGCGGGGCCAGAGGTAGTTGAGTGGTTGGCTAGCG440.4320078546882671No Hit
ATTGCGCAATGCCTCGCGTGGGGGGCCCCCTCCAAACGGGGGGCTTTCAA430.4221894943544428No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG420.4123711340206186No Hit
ATTGCGCAATGTATAAACCGGGTGAGTGATCCTGTACTGTCGAGAAAAGC390.3829160530191458No Hit
ATTGCGCAATGCCGTCTCGGGGGAAGAAGGGGGACCGATGTTGGTGAGCA370.3632793323514973No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGC350.3436426116838488No Hit
ATTGCGCAATGGGATGCTCGGGGTTGTGGGTGAGTGAGTGATCCTGTACT340.3338242513500245No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT330.3240058910162003No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCAT310.3043691703485518No Hit
ATTGCGCAATGTAAACCTCGGGTGAAAAGTGAGCGGATGAACTGAGGGTA310.3043691703485518No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT280.27491408934707906No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT250.24545900834560627No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT240.23564064801178203No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA230.22582228767795776No Hit
ATTGCGCAATGATGAAGAGGGGAAGTTCATCAAGTCGGGTGACGCCGCCA210.2061855670103093No Hit
ATTGCGCAATGTATTTCAAGGGGAATCTTAGCGAAAGCGATTGGCATAAC200.19636720667648502No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG200.19636720667648502No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCG200.19636720667648502No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCAGCG200.19636720667648502No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA190.18654884634266078No Hit
ATTGCGCAATGAGGTGGACGGGGTTGTAGGGGCGCACGGTGGATGCCTGG190.18654884634266078No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC190.18654884634266078No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT180.17673048600883653No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA170.16691212567501226No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC160.15709376534118802No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC150.14727540500736377No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGC150.14727540500736377No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG150.14727540500736377No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG140.13745704467353953No Hit
ATTGCGCAATGTCGCCGCCGGGCGAGGAGTCCGAGGGAGGCGAATCGGCT140.13745704467353953No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG130.12763868433971526No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTG120.11782032400589101No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC110.10800196367206677No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGC110.10800196367206677No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.10800196367206677No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG110.10800196367206677No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG110.10800196367206677No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCAGCT110.10800196367206677No Hit
ATTGCGCAATGGGGTAACGGGGTACCCTAAACCGACACTGGTGGATAGGT110.10800196367206677No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT110.10800196367206677No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)