FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10185
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTA7627.48159057437408No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2392.346588119783996No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1271.24693176239568No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1241.2174766813942073No Hit
CTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGG850.8345606283750614No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.7560137457044673No Hit
CTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTC650.6381934216985763No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCA650.6381934216985763No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGGTCGCC550.5400098183603338Illumina Single End PCR Primer 1 (95% over 23bp)
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT480.47128129602356406No Hit
ATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGG420.4123711340206186No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.4123711340206186No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGG400.39273441335297005No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG390.3829160530191458No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTG380.37309769268532156No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG370.3632793323514973No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC330.3240058910162003No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG330.3240058910162003No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.27491408934707906No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.2650957290132548No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA260.2552773686794305No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG200.19636720667648502No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGGGCGCC200.19636720667648502No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC190.18654884634266078No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC180.17673048600883653No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC180.17673048600883653No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA170.16691212567501226No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGA170.16691212567501226No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC170.16691212567501226No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGGGGTCGCC160.15709376534118802No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGG160.15709376534118802No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA160.15709376534118802No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA150.14727540500736377No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC150.14727540500736377No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA140.13745704467353953No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGGGTA140.13745704467353953No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT140.13745704467353953No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA130.12763868433971526No Hit
CTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGTTCTCGG130.12763868433971526No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA120.11782032400589101No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA120.11782032400589101No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA110.10800196367206677No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATC110.10800196367206677No Hit
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.10800196367206677No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)