FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9316
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCG3683.9501932159725204No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCT2732.930442249892658No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2152.3078574495491626No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1671.7926148561614428No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC930.9982825246887076No Hit
ATTGCGCAATGAATCACCCGGGAGAACGTCGTGAGACAGTTCGGTCTCTA610.6547874624302276No Hit
ATTGCGCAATGTCGGGGTAGGGTTATCAGCAAGTGATATTGATAAAGTAT550.5903821382567626No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG520.55817947617003No Hit
ATTGCGCAATGGACCTAAGGGGAGAAGAGAGAATGCTGGCATGAGTAGCG500.536711034778875No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGC500.536711034778875No Hit
ATTGCGCAATGTCGGGGGAGGGTTATCAGCAAGTGATATTGATAAAGTAT460.49377415199656505No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAAT420.4508372692142551No Hit
ATTGCGCAATGCGGCTCCGGGGGTTCCTGCTTAAAGTTAATCTGAGCAGG380.40790038643194504No Hit
ATTGCGCAATGGCGTGGTAGGGATTCTGACGGAAATCTACCATATCGTGT370.39716616573636754No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC370.39716616573636754No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC360.38643194504079004No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG350.37569772434521254No Hit
ATTGCGCAATGCTCCACCTGGGGGGCGTCAAATGTTTATCATGTTAGCGC330.35422928295405753No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA320.34349506225848003No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT320.34349506225848003No Hit
ATTGCGCAATGGTTGCTTTGGGGGGCGTCAAATGTTTATCATGTTAGCGC300.32202662086732503No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT280.30055817947617003No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT270.2898239587805925No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA240.25762129669386No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCG230.24688707599828252No Hit
ATTGCGCAATGGGTACAGTGGGTACAGTACACCTGTAATCCCAACATTTT230.24688707599828252No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT210.22541863460712755No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCACTCG210.22541863460712755No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC210.22541863460712755No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC200.21468441391155002No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG180.19321597252039502No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA160.17174753112924002No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG150.16101331043366252No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC140.15027908973808501No Hit
ATTGCGCAATGCTGAGTGAGGGGAGGTGGGGGAAACTGAAATTGCGGGCA130.1395448690425075No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT120.12881064834693No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT120.12881064834693No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG120.12881064834693No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAAGCG120.12881064834693No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC110.11807642765135251No Hit
ATTGCGCAATGCATGATCAGGGGGGTCAAGTGAATAAGTGCATATGGTGG110.11807642765135251No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC110.11807642765135251No Hit
ATTGCGCAAAAAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGA110.11807642765135251No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT110.11807642765135251No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCGCG110.11807642765135251No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT100.10734220695577501No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGGTG100.10734220695577501No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.10734220695577501No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100.10734220695577501No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG100.10734220695577501No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCG100.10734220695577501No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG100.10734220695577501No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)