FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9316
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTA6767.256333190210391No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2262.4259338772005155No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1211.2988407041648777No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1171.2559038213825675No Hit
CTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGG961.03048518677544No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGGTCGCC710.7621296693860026Illumina Single End PCR Primer 1 (95% over 23bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.7191927866036926No Hit
CTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTC600.6440532417346501No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCA550.5903821382567626No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTG440.47230571060541005No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG420.4508372692142551No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT380.40790038643194504No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGG340.36496350364963503No Hit
ATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGG300.32202662086732503No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG270.2898239587805925No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.279089738085015No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC240.25762129669386No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA230.24688707599828252No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC220.23615285530270502No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGA210.22541863460712755No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC210.22541863460712755No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.22541863460712755No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.21468441391155002No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG170.18248175182481752No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC170.18248175182481752No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA160.17174753112924002No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA160.17174753112924002No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGGGTA160.17174753112924002No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA150.16101331043366252No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA150.16101331043366252No Hit
CTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGTTCTCGG150.16101331043366252No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGGGGTCGCC140.15027908973808501No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG140.15027908973808501No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT140.15027908973808501No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAG130.1395448690425075No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT130.1395448690425075No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA130.1395448690425075No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC120.12881064834693No Hit
GCTTTTAGAAACTAATTTAGCTTTACGTAATTTACGTTTTTGTTTAGTGT120.12881064834693No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGGGCGCC120.12881064834693No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGG120.12881064834693No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT110.11807642765135251No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC110.11807642765135251No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA110.11807642765135251No Hit
TTTTTTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA110.11807642765135251No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGT110.11807642765135251No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGTTCTCGGTGGTCGCC110.11807642765135251No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA110.11807642765135251No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA100.10734220695577501No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGGTCGGC100.10734220695577501Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)