FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12892
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCG4263.3043748060812907No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCT3602.792429413589823No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2822.1874030406453615No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2772.148619298789947No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2501.9391870927707104No Hit
ATTGCGCAATGAATGAGGAGGGGATAAGGGGGGATGGAAAAGAGAATAAA830.6438101147998759No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG740.5739993794601302No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC740.5739993794601302No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGAT640.49643189574930185No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGAT620.48091839900713623No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA590.45764815389388763No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT580.4498914055228048No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC580.4498914055228048No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT480.37232392181197643No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT480.37232392181197643No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA410.31802668321439653No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA380.294756438101148No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG370.2869996897300651No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC360.2792429413589823No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT340.26372944461681663No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG320.24821594787465093No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA290.2249457027614024No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC290.2249457027614024No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG280.21718895439031957No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA270.2094322060192367No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG260.20167545764815392No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAGAGATGATCG260.20167545764815392No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCG250.19391870927707106No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC250.19391870927707106No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC230.17840521253490538No Hit
ATTGCGCAATGCCATGCAGGGGCGTGGCGGCGACGACCCATTCGAACGTC210.16289171579273967No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAG210.16289171579273967No Hit
ATTGCGCAATGGAAGGTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAG210.16289171579273967No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGAGCG190.147378219050574No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG190.147378219050574No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGC190.147378219050574No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT170.13186472230840832No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAGAGATGATCT170.13186472230840832No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGA160.12410797393732546No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT160.12410797393732546No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT160.12410797393732546No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT160.12410797393732546No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG160.12410797393732546No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG150.11635122556624264No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT150.11635122556624264No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT150.11635122556624264No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.10859447719515979No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC140.10859447719515979No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCT130.10083772882407696No Hit
ATTGCGCAATGGGGCGTGTGGGATAAAAAAGAGAAACTAAAAAAAAAAAA130.10083772882407696No Hit
ATTGCGCAATGCTCATTATGGGGCGGATGTAGCTCAGTTGGCTAGAGCGC130.10083772882407696No Hit
ATTGCGCAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGA130.10083772882407696No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT130.10083772882407696No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)