FastQCFastQC Report
Tue 7 May 2024
EGAF00007822476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9951
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTACGTGTA3233.2459049341774695No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1811.8189126720932571No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1631.6380263290121595No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTACGTGGA1591.5978293638830268No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTACGTGGT1381.3867952969550799No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTACGTGTT1341.3465983318259471No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1051.0551703346397348No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.7637423374535223No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTAC690.6933976484775399No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTACGTG570.5728067530901417No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.4019696512913275No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAAT330.33162496231534516No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC310.3115264797507788No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.29142799718621243No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG270.27132951462164606No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC260.2612802733393629No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC250.2512310320570797No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC210.21103406692794696No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG200.20098482564566375No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA190.19093558436338057No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA190.19093558436338057No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG190.19093558436338057No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT170.1708371017988142No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC160.16078786051653102No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.16078786051653102No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTA150.1507386192342478No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTACGTGTG150.1507386192342478No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.14068937795196462No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA140.14068937795196462No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT140.14068937795196462No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA130.13064013666968144No Hit
CTTATACACATCTGACGCTGCCGACGACCAATGTTACGTGTAGATCTCGG130.13064013666968144No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG130.13064013666968144No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC130.13064013666968144No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG120.12059089538739826No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA120.12059089538739826No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAAT110.11054165410511506No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC110.11054165410511506No Hit
CCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAAT110.11054165410511506No Hit
TCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAAT110.11054165410511506No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC100.10049241282283188No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)