FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547
Sequences flagged as poor quality0
Sequence length151
%GC70

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT30919.97414350355527No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1117.175177763413057No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC613.94311570782159No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGGGGGGGGGG110.7110536522301228No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGGTGGTCGCC110.7110536522301228Illumina RNA PCR Primer (95% over 23bp)
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTGGGGGGGGGGG80.5171299288946347No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGGGGGGGGGGGGG70.4524886877828055No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTCGGGGG40.25856496444731736No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGGCGGGGG40.25856496444731736No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTGTGGGGGGGGGGG40.25856496444731736No Hit
GTGTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.25856496444731736No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGGTGG40.25856496444731736No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTCGGGGGGGGGG40.25856496444731736No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTATATCTCGGTGGTCGCC40.25856496444731736Illumina Single End PCR Primer 1 (95% over 21bp)
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.19392372333548805No Hit
TGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGC30.19392372333548805No Hit
GTGTATAAGAGACCGATTGCGCAATCTGTCTCTTATACACATCTGACGCT30.19392372333548805No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTCGGTGGGGGGG30.19392372333548805No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTGGTGTCGGGGGGGGGG30.19392372333548805No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTGGGTGGGGGGG30.19392372333548805No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTGGCGGGGGGGGGG30.19392372333548805No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGGGGGGGGGGGGG30.19392372333548805No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTTGATGTCGGGGG30.19392372333548805No Hit
GCCCCATCACCCAGAGCACAAATGGTGTGGCCTTCTATCTGCTTGCTGAT30.19392372333548805No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTAAACACATCTGACGCT30.19392372333548805No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTGGGGGG30.19392372333548805No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTCGGGGG30.19392372333548805No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTCGGGGGTGGGG20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTGGGGGGGGTGGGG20.12928248222365868No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTGTCGGGGG20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGGCGGGGGGGGGG20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGGCGGGGGGGGGC20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTTGTGTGGGGGGGGGGG20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTTTGGGGGGGGGGG20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTGTCGGGGGGGGGG20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTAGGGGGGGGGGGG20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGAGTTCTCGGGGGGGGGG20.12928248222365868No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGATGTGGGTGG20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTGTTGTGGGGGGGGGGC20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGAGTTGTGGGTGGGGGGG20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTGGTGTGGGTGGGGGGG20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGATGTGGGGGGGGGGC20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTCTCGGGGGGGGGG20.12928248222365868No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGCATACGATTTTTTTTTT20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTGCTCGGGGGGGGGC20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTGGGGGGGGGGGGG20.12928248222365868No Hit
TCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTCGGTGGGGGGGGG20.12928248222365868No Hit
TGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTG20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTGTTGTCGGGGGGGGGG20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTCGGGGGGGGGG20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTGTGGGGGGGGGGG20.12928248222365868No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACCATCTGACGCTGCC20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTCTCGGGGGGGGGC20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGGAGGGGGGGGGG20.12928248222365868No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGGGGGTGG20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGTGTGTCGGGGGGGGGG20.12928248222365868No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTTCGGTAT20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGGGTCGGGGGGGGGG20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGGCGGGGGGGGGG20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATAACGGTGGTCGCC20.12928248222365868No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATTT20.12928248222365868No Hit
ATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACG20.12928248222365868No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTCTGGGGGG20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATATCGGTGGTCGCC20.12928248222365868Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGATGACGGTGAGGTGTATATATCGGTGGTCGCC20.12928248222365868No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGGGGAGGGGG20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTGGTCTGGGGGGGGGGC20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTGTTCTCGGGGGGGGGC20.12928248222365868No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGGGGGGGG20.12928248222365868No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCACGGTGGTCGCC20.12928248222365868Illumina Single End PCR Primer 1 (95% over 21bp)
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGGGGG20.12928248222365868No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)