FastQCFastQC Report
Tue 7 May 2024
EGAF00007822481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8822
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCG3503.967354341419179No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCT2172.459759691679891No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1611.8249829970528226No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1041.1788710043074133No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC820.929494445703922No Hit
ATTGCGCAATGGCAGTAATGGGGGAGAGTTTGATCCTGGCTCAGGACGAA700.793470868283836No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTG430.487417819088642No Hit
ATTGCGCAATGAGTCTCTGGGGGTGTGAACCGATCATGTAGTAGGCAAGC400.4534119247336205No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG360.4080707322602585No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGC350.396735434141918No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCAT340.3854001360235774No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC300.3400589435502154No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT290.3287236454318749No Hit
ATTGCGCAATGCTGGCTACGGGGATACAACAACTACAGAATCATGACAAA270.3060530491951938No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA210.23804126048515076No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCG210.23804126048515076No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC210.23804126048515076No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT180.20403536613012924No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT160.18136476989344819No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC160.18136476989344819No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCAGCG160.18136476989344819No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC160.18136476989344819No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGC150.1700294717751077No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC140.1586941736567672No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.14735887553842666No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG130.14735887553842666No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT120.13602357742008614No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG120.13602357742008614No Hit
ATTGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.12468827930174563No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT110.12468827930174563No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT100.11335298118340513No Hit
ATTTCGCAATGGCAGTAATGGGGGAGAGTTTGATCCTGGCTCAGGACGAA100.11335298118340513No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGT100.11335298118340513No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC90.10201768306506462No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC90.10201768306506462No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGG90.10201768306506462No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG90.10201768306506462No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)