FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8372
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATCG2372.8308647873865262No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1702.030578117534639No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1651.970855231724797No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATCT1381.6483516483516485No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1301.5527950310559007No Hit
ATTGCGCAATGACATCAACGGGGAGTCTTCAAGAACAGCCATGTCGCCCG931.1108456760630674No Hit
ATTGCGCAATGCTTGCTACGGGGCTCTTCCTGTAAGCGGCCAGAGGTCAC810.9675107501194458No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATCG650.7763975155279503No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACAT420.5016722408026756No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG380.4538939321548017No Hit
ATTGCGCAATGTCATGCCTGGGGGTGCGGGCGTATAGACGAACAATCTTG370.4419493549928333No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC350.4180602006688963No Hit
ATTGCGCAATGAGAGCGGTGGGCAGACCACTACAAGCGCCTGCTGAGGAA340.40611562350692787No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT330.3941710463449594No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC330.3941710463449594No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT310.37028189202102246No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAG300.358337314859054No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT260.3105590062111801No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC220.26278069756330624No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAG220.26278069756330624No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCAT180.21500238891543239No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC160.19111323459149546No Hit
ATTGCGCAATGGGCGAAGCGGGAGGGCTGGTGAGATGGCTCAGTGGTTAA160.19111323459149546No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG150.179168657429527No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG150.179168657429527No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATCT150.179168657429527No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT150.179168657429527No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA140.16722408026755853No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT140.16722408026755853No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCAGCG130.15527950310559005No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACA120.1433349259436216No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG120.1433349259436216No Hit
ATTGCGCAATGGAAGGCCGGGGAAGGAGTGAGGCCCAGCCCCATGCAGCT120.1433349259436216No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAA120.1433349259436216No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG110.13139034878165312No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT110.13139034878165312No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG110.13139034878165312No Hit
ATTGCGCAATGGGACAGGCGGGGAAGGAGTGAGGCCCAGCCCCATGCAGC110.13139034878165312No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA110.13139034878165312No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT100.11944577161968467No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG100.11944577161968467No Hit
ATTGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATC100.11944577161968467No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT100.11944577161968467No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGG90.10750119445771619No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT90.10750119445771619No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTG90.10750119445771619No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.10750119445771619No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATGG90.10750119445771619No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGG90.10750119445771619No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG90.10750119445771619No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)