FastQCFastQC Report
Tue 7 May 2024
EGAF00007822496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4233
Total Bases639.1 kbp
Sequences flagged as poor quality0
Sequence length151
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT59814.127096621781243No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2756.496574533427829No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC491.1575714623198678No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGT350.8268367587999055No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTT240.5669737774627923No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.42523033309709424No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAG180.42523033309709424No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACG150.3543586109142452No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTA140.3307347035199622No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC130.3071107961256792No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG110.25986298133711316No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGA110.25986298133711316No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTG100.23623907394283014No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC90.21261516654854712No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT80.18899125915426412No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.18899125915426412No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGGTGGTCGCC80.18899125915426412Illumina RNA PCR Primer (95% over 23bp)
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGGGGGGGGGGGGG70.1653673517599811No Hit
ACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTT70.1653673517599811No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA60.14174344436569808No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC60.14174344436569808No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG60.14174344436569808No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGG60.14174344436569808No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTC60.14174344436569808No Hit
ATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG60.14174344436569808No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGGGGGGGGGG60.14174344436569808No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA60.14174344436569808No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTG60.14174344436569808No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTGGGGGGGGGGG50.11811953697141507No Hit
AGCTACACTTGGCCACAAGAGAACAGAGCTAGTCCAGAGTGCCGGGGGTC50.11811953697141507No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGGGGGGGGGGGGG50.11811953697141507No Hit
TGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTG50.11811953697141507No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTCGGGGGGGGGG50.11811953697141507No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTTGTTGTGGG50.11811953697141507No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC50.11811953697141507No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC50.11811953697141507No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA50.11811953697141507No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTGTG50.11811953697141507No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTCGG50.11811953697141507No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)