FastQCFastQC Report
Tue 7 May 2024
EGAF00007822500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6928
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGG3324.7921478060046185No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1712.46824480369515No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1191.7176674364896074No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGT1051.5155889145496535No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.8660508083140877No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.7650115473441108No Hit
CATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGGGGGGGGGGGGG380.5484988452655889No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAG360.5196304849884527No Hit
CTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGGGGG350.5051963048498845No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTG330.47632794457274824No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGTG310.44745958429561206No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGTA250.3608545034642032No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT230.331986143187067No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.3175519630484988No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC200.28868360277136257No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC200.28868360277136257No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA200.28868360277136257No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.27424942263279445No Hit
ATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGGGGGGGG180.25981524249422633No Hit
CTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGGG170.2453810623556582No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGTT170.2453810623556582No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG170.2453810623556582No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG160.23094688221709006No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA160.23094688221709006No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG160.23094688221709006No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC150.2165127020785219No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG150.2165127020785219No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGAT140.20207852193995382No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGA140.20207852193995382No Hit
CATCTGACGCTGCCGACGATCGATATGAGGTGGGGGTGGGGGGGGGGGGG140.20207852193995382No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.17321016166281755No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC120.17321016166281755No Hit
CATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGTGGGGGGGGGGG120.17321016166281755No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA110.1587759815242494No Hit
CTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGTGGGGG110.1587759815242494No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC100.14434180138568128No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.12990762124711316No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA90.12990762124711316No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGG90.12990762124711316No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA90.12990762124711316No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC90.12990762124711316No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA90.12990762124711316No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA80.11547344110854503No Hit
CTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGTGGG80.11547344110854503No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA80.11547344110854503No Hit
CTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGTGGGG80.11547344110854503No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT80.11547344110854503No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGA80.11547344110854503No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC80.11547344110854503No Hit
GCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCGCTGAGGAG70.10103926096997691No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT70.10103926096997691No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA70.10103926096997691No Hit
CTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGTGTGGG70.10103926096997691No Hit
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.10103926096997691No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA70.10103926096997691No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGA70.10103926096997691No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA70.10103926096997691No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGA70.10103926096997691No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA70.10103926096997691No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)