FastQCFastQC Report
Tue 7 May 2024
EGAF00007822502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7356
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGG3975.396954866775421No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1772.4061990212071778No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGT1261.7128874388254487No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC941.277868406742795No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT761.0331702011963024No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAG510.6933115823817292No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.6525285481239804No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.5709624796084829No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGTG400.5437737901033171No Hit
CATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGGGGGGGGGGGGG400.5437737901033171No Hit
CTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGGGGG360.48939641109298526No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTG330.4486133768352365No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT330.4486133768352365No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG330.4486133768352365No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGTA310.42142468733007066No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.42142468733007066No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC270.367047308319739No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG220.29907558455682437No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGTT220.29907558455682437No Hit
ATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGGGGGGGG210.28548123980424145No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA210.28548123980424145No Hit
CATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGTGGGGGGGGGGG210.28548123980424145No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGAT200.27188689505165853No Hit
CATCTGACGCTGCCGACGATCGATATGAGGTGGGGGTGGGGGGGGGGGGG180.24469820554649263No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG160.2175095160413268No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC150.20391517128874387No Hit
CTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGGG140.19032082653616098No Hit
CTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGTGGG130.17672648178357803No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC130.17672648178357803No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGA120.1631321370309951No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA120.1631321370309951No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA120.1631321370309951No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1631321370309951No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGA120.1631321370309951No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC120.1631321370309951No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGG110.14953779227841218No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA110.14953779227841218No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA110.14953779227841218No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG100.13594344752582926No Hit
CTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGTGGGGG100.13594344752582926No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC100.13594344752582926No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC90.12234910277324632No Hit
ATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGTGGGGGGGG90.12234910277324632No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA90.12234910277324632No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA90.12234910277324632No Hit
CATCTCCCTCGTAGCCAGCCACAGGAGCATTGTTCAATCTGATGACCACG90.12234910277324632No Hit
CATCTGACGCTGCCGACGATCGATATGAGGTGTAGATCTCGGTGGTCGCC90.12234910277324632Illumina RNA PCR Primer (95% over 23bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC90.12234910277324632No Hit
CAGGTATAGAATAGCTAGTGATGGCCAGCACCCGGAGAAGAAGGTCTTCA80.1087547580206634No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG80.1087547580206634No Hit
GGTATAGAATAGCTAGTGATGGCCAGCACCCGGAGAAGAAGGTCTTCACT80.1087547580206634No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)