FastQCFastQC Report
Tue 7 May 2024
EGAF00007822507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8171
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCG3143.842858891200587No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCT2352.8760249663443886No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1732.1172439113939543No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1181.4441316852282462No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC831.015787541304614No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGC530.6486354179415005No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG370.45282095214783996No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG330.4038673356994248No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAAT300.3671521233631134No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC300.3671521233631134No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT230.281483294578387No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG210.2570064863541794No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC210.2570064863541794No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT200.24476808224207564No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCACTCG190.23252967812997186No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC190.23252967812997186No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCG190.23252967812997186No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC180.22029127401786805No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA180.22029127401786805No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG160.19581446579366052No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAAGCG160.19581446579366052No Hit
ATTGCGCAATGTGTACCCGGGGGGGTTGATGGTGGTGATCCTGTACTGTC150.1835760616815567No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA150.1835760616815567No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT140.17133765756945293No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCT140.17133765756945293No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT140.17133765756945293No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT130.15909925345734915No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC130.15909925345734915No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC120.14686084934524538No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA120.14686084934524538No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG120.14686084934524538No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC110.1346224452331416No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT110.1346224452331416No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG110.1346224452331416No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATGG110.1346224452331416No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCGCG100.12238404112103782No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCACTCT90.11014563700893402No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC90.11014563700893402No Hit
ATTGCGCAATGCTCGTCCGGGGCAAGGGTAACCGCTCCTGAATATATAGG90.11014563700893402No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG90.11014563700893402No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG90.11014563700893402No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAAGCT90.11014563700893402No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT90.11014563700893402No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)