FastQCFastQC Report
Tue 7 May 2024
EGAF00007822509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10065
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCG3713.6860407352210633No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCT3283.2588176850471933No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3093.070044709388972No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2032.016890213611525No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1361.351217088922007No Hit
ATTGCGCAATGGCGGCAGGGGGGTGACAATGAGGTCCAACGCACGATGCT710.7054148037754595No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTA700.6954793840039741No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC670.6656731246895181No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG520.5166418281172379No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT400.39741679085941384No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC400.39741679085941384No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAAT380.37754595131644314No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTG370.36761053154495776No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT340.33780427223050175No Hit
ATTGCGCAATGTGGTTCTTGGGGGGCCGGGTGCGGTGGCTCACTCCTGTA330.32786885245901637No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC290.28812717337307503No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA290.28812717337307503No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT290.28812717337307503No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA240.23845007451564829No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCT220.2185792349726776No Hit
ATTGCGCAATGCTTCTATCGGGGAACGTCCAGCAGAACTATCATGCCTTC220.2185792349726776No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC210.20864381520119224No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGC200.19870839542970692No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTG200.19870839542970692No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCG190.18877297565822157No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC170.16890213611525087No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT170.16890213611525087No Hit
ATTGCGCAATGATGGAGTGGGGGAGGAGCGGAGGACGCTGGCGCTGCAGC160.15896671634376552No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG160.15896671634376552No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA150.14903129657228018No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGGTAAATCG140.13909587680079483No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCGCG130.12916045702930948No Hit
ATTGCGCAATGATCCTAGGGGGTGGATAGGTAGAGTATACCGAGGCGATC130.12916045702930948No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGT130.12916045702930948No Hit
CGCCTGTAATCCCACAACTCTGGGAGACCGAGGCCGGTGGATCACCTGAG120.11922503725782414No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGGTAAATCT120.11922503725782414No Hit
ATTGCGCAATGTATGCTGCTGCTGTCTCTTATACACATCTCCGAGCCCAC120.11922503725782414No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT120.11922503725782414No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGG120.11922503725782414No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT120.11922503725782414No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTT120.11922503725782414No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT120.11922503725782414No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.1092896174863388No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC110.1092896174863388No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT110.1092896174863388No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC110.1092896174863388No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG110.1092896174863388No Hit
ATTCTGGACTGACTGACTGACTGAAATAAAGTGTTCTGTTTTGCTGTTTT110.1092896174863388No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)