FastQCFastQC Report
Tue 7 May 2024
EGAF00007822512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2693
Total Bases406.6 kbp
Sequences flagged as poor quality0
Sequence length151
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT46117.118455254363163No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2168.020794652803565No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC592.1908652060898626No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTA130.4827330115113257No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGGTGGTCGCC90.33419977720014854Illumina RNA PCR Primer (95% over 23bp)
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGGGGGGGGGG70.25993316004455996No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGT70.25993316004455996No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.22279985146676567No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTGGGGGGGGGGG60.22279985146676567No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGG50.18566654288897141No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTC50.18566654288897141No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAG50.18566654288897141No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGA50.18566654288897141No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTTGATGTCGG40.14853323431117713No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA40.14853323431117713No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTCGGGGGGGGGG40.14853323431117713No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGGCGGGGGGGGGG40.14853323431117713No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG40.14853323431117713No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGGGGGGGGGG30.11139992573338284No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGAGTTGTCGGGGGGGGGG30.11139992573338284No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTGTGGG30.11139992573338284No Hit
TGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTG30.11139992573338284No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTAGTTCTC30.11139992573338284No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTG30.11139992573338284No Hit
GGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTAC30.11139992573338284No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTGGCGGGGGGGGGG30.11139992573338284No Hit
ATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG30.11139992573338284No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGGGGGGGGGC30.11139992573338284No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGATGGCGGGGGGGGGG30.11139992573338284No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTT30.11139992573338284No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.11139992573338284No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGGTGG30.11139992573338284No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGGTGGGGGGG30.11139992573338284No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGA30.11139992573338284No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGG30.11139992573338284No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)