FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10413
Sequences flagged as poor quality0
Sequence length151
%GC60

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC6306.050129645635264No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC4023.860558916738692No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCG1241.1908191683472582No Hit
ATTGCGCAATGGACACCGGGGGCGGACGTTGAGACCAGGATTTCCGAATG1111.0659752232785942No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCG1071.027561701719005No Hit
ATTGCGCAATGAACACAGTGGGGGTCACAAGACCACTAAAATCGAGAGGG790.7586670508018822No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCT670.6434264861231154No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.5377893018342457No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCAC500.48016901949486224No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCT420.40334197637568425No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG400.38413521559588976No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCT390.37453183520599254No Hit
ATTGCGCAATGACGGGAGTGGGCGGATCACCTGAAGTCAGGAGTTTGATA380.36492845481609526No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAAT350.33611831364640354No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC260.24968789013732834No Hit
ATTGCGCAATGGGCCAACCGGGGAGGCAAGCGCATTAACAGGTGTGACGC260.24968789013732834No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG250.24008450974743112No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC250.24008450974743112No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT230.22087774896763662No Hit
ATTGCGCAATGATCTAGGGGGGAAGTTTCCAAGGGCGCATGGTGGATGCC210.20167098818784213No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC210.20167098818784213No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA180.17286084701815038No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAAGCG180.17286084701815038No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT170.16325746662825313No Hit
ATTGCGCAATGGCAATTACGGGGTCCAAGGACTCCAATTAAAAATTAATA160.15365408623835589No Hit
ATCTGTGGCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCG150.14405070584845864No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCT150.14405070584845864No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG150.14405070584845864No Hit
ATTGCGCAATGGGCAGCCTGGGGGAGCATTAGTGCCCGTTGCATCACCGC140.13444732545856142No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA130.12484394506866417No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATGG130.12484394506866417No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA130.12484394506866417No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCA130.12484394506866417No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT130.12484394506866417No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAAGCG120.11524056467876692No Hit
ATTGCGCAATGCGGGGGGGGGGGAAAACTAACGTTTTCAAGCTTTTTATT120.11524056467876692No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC120.11524056467876692No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC120.11524056467876692No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATGG110.10563718428886967No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)