FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11598
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCG5474.716330401793413No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCT4033.474737023624763No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2522.172788411795137No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2291.9744783583376442No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1891.6295913088463527No Hit
ATTGCGCAATGCCTCTAGTGGGAGGGGAGCGTTCTGTAAGCCGATGAAGG1631.4054147266770134No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG800.6897740989825831No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTC730.6294188653216072No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC690.594930160372478No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAAT620.534574926711502No Hit
ATTGCGCAATGGGTCATAGGGGGAAGCTGCGCAAGGTCCAGCTATACAGG570.49146404552509054No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA550.4742196930505259No Hit
ATTGCGCAATGTTGGGGGGGGGAGTGGTGAGGGCAGAGGAACCCTAAAGA490.4224866356268322No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT460.39662010691498534No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT440.37937575444042076No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC340.29315399206759785No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT330.28453181583031556No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG300.2586652871184687No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC300.2586652871184687No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA290.2500431108811864No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC250.21555440593205724No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAAGCG240.20693222969477496No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCG240.20693222969477496No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT210.1810657009829281No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT210.1810657009829281No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC190.1638213485083635No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA190.1638213485083635No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGT190.1638213485083635No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC180.1551991722710812No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT170.14657699603379892No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG170.14657699603379892No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCCATCG170.14657699603379892No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG170.14657699603379892No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG160.13795481979651664No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG150.12933264355923435No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA150.12933264355923435No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC150.12933264355923435No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCGGT150.12933264355923435No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC140.12071046732195206No Hit
ATTGCGCAATCGCGGTCCCGGGATGGTGGCCACGGCCACAGCCACTAATC130.11208829108466976No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAG130.11208829108466976No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT130.11208829108466976No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC130.11208829108466976No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCT130.11208829108466976No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG120.10346611484738748No Hit
ATTGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10346611484738748No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT120.10346611484738748No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC120.10346611484738748No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT120.10346611484738748No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCGCG120.10346611484738748No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATGG120.10346611484738748No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)