FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10447
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGG4914.699913850866277No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2782.6610510194314156No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1721.6464056666985738No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGT1361.3018091318081746No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1111.0625059825787309No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTT750.7179094476883315No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.6413324399349095No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGTG640.6126160620273763No Hit
CTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGGGG510.48817842442806547No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGTT490.46903417248970997No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGTA420.4020292907054657No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTG420.4020292907054657No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATT410.39245716473628794No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC350.33502440892122143No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.33502440892122143No Hit
CATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGGGGGGGGGGGG340.32545228295204365No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGGGGG310.2967359050445104No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC310.2967359050445104No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG300.28716377907533264No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA280.26801952713697713No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.2584474011677994No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC230.22015889729108834No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC230.22015889729108834No Hit
CTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGG220.21058677132191062No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG220.21058677132191062No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG210.20101464535273286No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG180.17229826744519958No Hit
ATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGGGGGGG180.17229826744519958No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC180.17229826744519958No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGGGGT150.14358188953766632No Hit
CTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGTG150.14358188953766632No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC150.14358188953766632No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA140.13400976356848857No Hit
CCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATT140.13400976356848857No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG140.13400976356848857No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATT130.12443763759931081No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATT130.12443763759931081No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA130.12443763759931081No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT130.12443763759931081No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.12443763759931081No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.11486551163013305No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA120.11486551163013305No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC120.11486551163013305No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA120.11486551163013305No Hit
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.11486551163013305No Hit
CATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGTGGGGGGGGGGGGG120.11486551163013305No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGA110.10529338566095531No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC110.10529338566095531No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT110.10529338566095531No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)