FastQCFastQC Report
Tue 7 May 2024
EGAF00007822523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13422
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATCG5123.814632692594248No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATCT3082.2947399791387277No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2932.1829831619728806No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2341.743406347787215No Hit
ATTGCGCAATGACTGTCACGGGGGATCAAGCGACTAAGTGCATATGGTGG1851.378334078378781No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1741.2963790791238265No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACAT940.7003427209059753No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG650.4842795410520042No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCAT610.45447772314111157No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATCG610.45447772314111157No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAG600.44702726866338843No Hit
ATTGCGCAATGCGGGCCTCGGGGAATTACTAGTAATCGTGTATCACCAAC550.40977499627477276No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC540.4023245417970496No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC510.3799731783638802No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT450.33527045149754137No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT440.32781999701981823No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAG350.26076590672030997No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT330.24586499776486367No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT310.23096408880941738No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC310.23096408880941738No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG300.22351363433169422No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA290.2160631798539711No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATCT280.20861272537624795No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA280.20861272537624795No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA260.19371181642080165No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT220.16390999850990912No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG220.16390999850990912No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC220.16390999850990912No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG220.16390999850990912No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCAGCG180.13410818059901655No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG180.13410818059901655No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCAGCT170.1266577261212934No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT170.1266577261212934No Hit
ATTGCGCAATCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA170.1266577261212934No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC160.11920727164357026No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT160.11920727164357026No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGG150.11175681716584711No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAG140.10430636268812397No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG140.10430636268812397No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATCGCG140.10430636268812397No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)