FastQCFastQC Report
Tue 7 May 2024
EGAF00007822524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13422
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGG5293.9412904187155413No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2742.0414245268961406No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2141.5943972582327524No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1881.4006854418119505No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGT1621.206973625391149No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTT1020.7599463567277605No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.737594993294591No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGTG910.6779913574728059No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC590.43957681418566535No Hit
CATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGGGGGGGGGGGG580.4321263597079422No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTG560.4172254507524959No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATT540.4023245417970496No Hit
CTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGGGG520.3874236328416033No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGTT510.3799731783638802No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGGGGG460.3427209059752645No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGTA400.29801817910892564No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.2905677246312025No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.2831172701534794No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATT360.2682163611980331No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG340.2533154522425868No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.24586499776486367No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC310.23096408880941738No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG300.22351363433169422No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC300.22351363433169422No Hit
CTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGG290.2160631798539711No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA280.20861272537624795No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC280.20861272537624795No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC280.20861272537624795No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG260.19371181642080165No Hit
ATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGGGGGGG260.19371181642080165No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG260.19371181642080165No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA230.17136045298763225No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC220.16390999850990912No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT200.14900908955446282No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC180.13410818059901655No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA180.13410818059901655No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGG170.1266577261212934No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATT150.11175681716584711No Hit
CATCTGACGCTGCCGACGACCATTGTCTTGGGGGGGGGGGGGGGGGGGGG150.11175681716584711No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC150.11175681716584711No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA150.11175681716584711No Hit
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.11175681716584711No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.10430636268812397No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)