FastQCFastQC Report
Tue 7 May 2024
EGAF00007822529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13493
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCT5684.209590157859631No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCG5404.002075150077818No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3192.3641888386570815No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3132.31972133698955No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2231.6527088119765803No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG990.7337137775142667No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTA850.6299562736233603No Hit
ATTGCGCAATGGAAGACAGGGGGGGGCAGGAAGAAAGCAAACGGAGTAAG760.5632550211220633No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAAT740.5484325205662196No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC640.4743200177870006No Hit
CTGTGGTCCTGGGTGGCTGGGCGCGGTGGCTCACGCCTGTAATCCCAGCA570.42244126584154745No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTG490.3631512636181724No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT490.3631512636181724No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA440.32609501222856296No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT420.3112725116727192No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG370.2742162602831098No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC360.26680501000518786No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCG340.25198250944934414No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT320.2371600088935003No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT310.22974875861557845No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG310.22974875861557845No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG300.2223375083376566No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC300.2223375083376566No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC290.2149262580597347No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTT270.2001037575038909No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT260.192692507225969No Hit
ATTGCGCAATGCACATTCCGGGGGAACTCGGCAAAATGCACGCGTAACTT260.192692507225969No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCT250.18528125694804715No Hit
ATTGCGCAATGGATGTCTGGGGGGTGCAAGCTCTTGATTGAAGTCCCAGT250.18528125694804715No Hit
CTGTGGACCTGGGTGGCTGGGCGCGGTGGCTCACGCCTGTAATCCCAGCA220.16304750611428148No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA190.14081375528051582No Hit
ATTGCGCAATGGAACTTCTGGGGGGGCAGGAAGAAAGCAAACGGAGTAAG190.14081375528051582No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT180.13340250500259393No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC170.12599125472467207No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGGTAAATCG170.12599125472467207No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC170.12599125472467207No Hit
ATGTGGTCCTGGGTGGCTGGGCGCGGTGGCTCACGCCTGTAATCCCAGCA170.12599125472467207No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.11858000444675015No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG160.11858000444675015No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT150.1111687541688283No Hit
ATTGCGCAATGCTCGGGGGGGTGGAGGGCTGGGGGAGGGATAGCATTAGG150.1111687541688283No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC150.1111687541688283No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG150.1111687541688283No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGC150.1111687541688283No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG150.1111687541688283No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT150.1111687541688283No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGC140.10375750389090639No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCGCG140.10375750389090639No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT140.10375750389090639No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGATG140.10375750389090639No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)