FastQCFastQC Report
Tue 7 May 2024
EGAF00007822531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15485
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCT5963.8488860187278013No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCG5683.6680658701969646No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4172.6929286406199546No Hit
ATTGCGCAATGCGGTCGAGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTAC3252.098805295447207No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2891.8663222473361318No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2261.45947691314175No Hit
ATTGCGCAATGTTTAGGTCGGGCAAAAAAGTGAAACTTGGAGAATATCAA1030.6651598320955764No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG990.6393283823054569No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGAT980.6328705198579271No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGAT920.5941233451727478No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC840.5424604455925088No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA830.5360025831449791No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT770.4972554084597998No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC710.4585082337746206No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT600.38747174685179203No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT600.38747174685179203No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC380.245398773006135No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAGAGATGATCT380.245398773006135No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG370.2389409105586051No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC360.23248304811107523No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA350.22602518566354535No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAGAGATGATCG350.22602518566354535No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC340.2195673232160155No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA340.2195673232160155No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG340.2195673232160155No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT330.21310946076848564No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA300.19373587342589602No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA300.19373587342589602No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG280.18082014853083628No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG250.16144656118824668No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG250.16144656118824668No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCGCG240.15498869874071683No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT230.14853083629318695No Hit
ATTGCGCAATGACCTTGCTGGGAAGTAAGCTGTGATCCAGAGATTTCCGA220.1420729738456571No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT220.1420729738456571No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCG210.1356151113981272No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGA210.1356151113981272No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT210.1356151113981272No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAG210.1356151113981272No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.12915724895059735No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG190.1226993865030675No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT190.1226993865030675No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG190.1226993865030675No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGAGCG180.11624152405553762No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGAGCT180.11624152405553762No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT180.11624152405553762No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC170.10978366160800775No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGAGGATCG160.10332579916047789No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT160.10332579916047789No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCTCG160.10332579916047789No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)