FastQCFastQC Report
Tue 7 May 2024
EGAF00007822532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15485
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGG7714.979011947045528No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3592.3183726186632225No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2851.8404907975460123No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGT2101.3561511139812723No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1981.2786567646109137No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.8847271553115919No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGTG1210.781401356151114No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTT1110.7168227316758152No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATT890.5747497578301581No Hit
CATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGGGGGGGGGGGG830.5360025831449791No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTG810.5230868582499193No Hit
CTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGGGG810.5230868582499193No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGGGGG790.5101711333548595No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGTT780.5037132709073296No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC690.4455925088795608No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGTA640.41330319664191156No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG620.4003874717468518No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.3939296092993219No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG480.30997739748143366No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA460.2970616725863739No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.290603810138844No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC440.2841459476913142No Hit
CTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGG440.2841459476913142No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.2647723603487246No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG400.2583144979011947No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC350.22602518566354535No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC350.22602518566354535No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA340.2195673232160155No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT340.2195673232160155No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATT320.20665159832095578No Hit
ATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGGGGGGG320.20665159832095578No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC320.20665159832095578No Hit
CCACTTCGGGCTCCACGGGCGTCTTTCCGGTATCTTTAAATGCCATGGCT280.18082014853083628No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC260.16790442363577657No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA240.15498869874071683No Hit
CTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGTGGG240.15498869874071683No Hit
CATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGTGGGGGGGGGGGGG230.14853083629318695No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCT220.1420729738456571No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA220.1420729738456571No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGG220.1420729738456571No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG210.1356151113981272No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAT210.1356151113981272No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC210.1356151113981272No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA200.12915724895059735No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC200.12915724895059735No Hit
CTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGTG190.1226993865030675No Hit
CATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGTGGGGGGGGGGG190.1226993865030675No Hit
CTTATACACATCTGACGCTGCCGACGACCATTGTCTTGGGGGGGGGGGGG180.11624152405553762No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGGGGT180.11624152405553762No Hit
CTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGTGGGGG180.11624152405553762No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGA170.10978366160800775No Hit
TCACTTTCAGATTCTTTTCCTTGGCGCCTCTGATCAAGTCCGCACAAACC160.10332579916047789No Hit
CATCTGACGCTGCCGACGACCATTGTCTTGGGGGGGGGGGGGGGGGGGGG160.10332579916047789No Hit
GATTCTTTTCCTTGGCGCCTCTGATCAAGTCCGCACAAACCTTCTCCAAC160.10332579916047789No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA160.10332579916047789No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA160.10332579916047789No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)